iTRAQ-based quantitative proteomic analysis of submandibular glands from rats with STZ-induced hyperglycemia

• Type: Article in International Scientific Journal
• Date: 2013

Authors

alves, rmp (Author) Other; vitorino, r (Author) Other; padrao, ai (Author) Other; moreira-goncalves, d (Author) Other; duarte, ja (Author) FADEUP; ferreira, rmp (Author) Other; amado, f (Author) Other

Journal

Title: Journal of Biochemistry

Vol. 153

Pages: 209-220

ISSN: 0021-924X

Publisher: Oxford University Press

Indexing

ISI Web of Knowledge - 5 Citations

Scopus - 4 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-002-0ZE

DOI: 10.1093/jb/mvs142

Abstract (EN): The impairment of salivary glands activity is often connected to the complaints of dry-mouth and subsequent degradation of the periodontium of diabetic patients. In this context, submandibular glands (SMGs) play a central role in saliva production and so the understanding of the molecular pathways affected is of paramount importance. Using a streptozotocin-induced hyperglycemia rat model and two different time points (2 and 4 months), we applied mass spectrometry-based proteomic techniques, validated with standard western blot analysis, to identify and quantify the effect of chronic hyperglycemia on the proteome of SMGs. We observed significant variations of proteins such as kallikreins, protein S100A6 or annexins. After 2 months of hyperglycemia, we observed an early phase response characterized by a significant increase of protein S100A6, linked to the inflammatory response, together with the impairment of metabolic and energy production processes. On the other hand, vesicular transport appeared to be favoured in such conditions. Interestingly, in a long-term response to hyperglycemia after 4 months of exposure, we observed a general attenuation of the variations. In conclusion, we present data that support the existence of an adaptation of the gland to long-term stress.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 12