Telomerase expression and proliferative activity suggest a stem cell role for thyroid solid cell nests

• Type: Article in International Scientific Journal
• Date: 2004

Authors

Preto, A (Author) Other; Cameselle Teijeiro, J (Author) Other; Moldes Boullosa, J (Author) Other; Soares, P (Author) FMUP; Cameselle Teijeiro, JF (Author) Other; Silva, P (Author) Other; Reis, JS (Author) Other; Reyes Santias, RM (Author) Other; Alfonsin Barreiro, N (Author) Other; Forteza, J (Author) Other; Sobrinho Simoes, M (Author) FMUP

Journal

Title: Modern Pathology

Vol. 17

Pages: 819-826

ISSN: 0893-3952

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 52 Citations

Scopus - 60 Citations

Scientific classification

FOS: Medical and Health sciences > Basic medicine

Other information

Authenticus ID: P-000-9SZ

DOI: 10.1038/modpathol.3800124

Abstract (EN): Solid cell nests of the human thyroid gland are composed of main cells and C cells. In order to investigate the putative stem cell nature of the role for solid cell nests, we evaluated the histological features, and the immunohistochemical expression of p63, bcl-2, telomerase catalytic subunit, and two proliferative markers (Ki-67 and minichromosome maintenance protein 2), in a series of 24 cases of solid cell nests. Proliferative indices were determined in (a) solid cell nests, (b) thyroid follicular cells in the vicinity of solid cell nests within a low-power field, and (c) distant thyroid tissue, at a distance of at least three low-power fields from solid cell nests. In 15 cases of solid cell nests (62.5%), mixed follicles were observed; papillary formations were observed in four cases (16.6%), and ciliated cells were observed in the lining of microcysts associated with two cases (8.3%). Salivary gland-type tissue, cartilage islands, adipose and fibrous tissues, and small nerves were also associated with some cases of solid cell nests. We observed that the main cells of the solid cell nests express consistently telomerase, although at lower levels than p63, and show strong cytoplasmic immunoreactivity for bcl-2, which is associated with an increased differentiation potential. We also observed that despite their relative low proliferative index, main cells of the solid cell nests display higher proliferation than follicular cells in the vicinity and follicular cells in more distant thyroid tissue. We conclude that main cells of the solid cell nests apparently harbor the minimal properties of a stem cell phenotype (capacity for both self-renewal, conferred by telomerase activity, and differentiation to one or more than one type of specialized cells, given by the high expression of p63 and bcl-2) and may thus represent a pool of stem cells of the adult thyroid.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8