Synergistic impact of endurance training and intermittent hypobaric hypoxia on cardiac function and mitochondrial energetic and signaling

• Type: Article in International Scientific Journal
• Date: 2013

Authors

magalhaes, j (Author) FADEUP; falcao-pires, i (Author) FMDUP; goncalves, io (Author) Other; lumini-oliveira, j (Author) Other; marques-aleixo, i (Author) Other; dos passos, e (Author) Other; rocha-rodrigues, s (Author) Other; machado, ng (Author) Other; moreira, ac (Author) Other; miranda-silva, d (Author) Other; moura, c (Author) Other; leite-moreira, af (Author) FMUP; oliveira, pj (Author) Other; torrella, jr (Author) Other; ascensao, a (Author) FADEUP

Journal

Title: International Journal of Cardiology

Vol. 168

Pages: 5363-5371

ISSN: 0167-5273

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 24 Citations

Scopus - 25 Citations

Other information

Authenticus ID: P-008-G84

DOI: 10.1016/j.ijcard.2013.08.001

Abstract (EN): Background Intermittent hypobaric-hypoxia (IHH) and endurance-training (ET) are cardioprotective strategies against stress-stimuli. Mitochondrial modulation appears to be an important step of the process. This study aimed to analyze whether a combination of these approaches provides additive or synergistic effects improving heart-mitochondrial and cardiac-function. Methods Two-sets of rats were divided into normoxic-sedentary (NS), normoxic-exercised (NE, 1 h/day/5 weeks treadmill-running), hypoxic-sedentary (HS, 6000 m, 5 h/day/5 weeks) and hypoxic-exercised (HE) to study overall cardiac and mitochondrial function. In vitro cardiac mitochondrial oxygen consumption and transmembrane potential were evaluated. OXPHOS subunits and ANT protein content were semi-quantified by Western blotting. HIF-1¿, VEGF, VEGF-R1 VEGF-R2, BNP, SERCA2a and PLB expressions were measured by qRT-PCR and cardiac function was characterized by echocardiography and hemodynamic parameters. Results Respiratory control ratio (RCR) increased in NE, HS and HE vs. NS. Susceptibility to anoxia/reoxygenation-induced dysfunction decreased in NE, HS and HE vs. NS. HS decreased mitochondrial complex-I and -II subunits; however HE completely reverted the decreased content in complex-II subunits. ANT increased in HE. HE presented normalized ventricular-arterial coupling (Ea) and BNP myocardial levels and significantly improved myocardial performance as evaluated by increased cardiac output and normalization of the Tei index vs. HS. Conclusion Data demonstrates that IHH and ET confer cardiac mitochondria with a more resistant phenotype although without visible addictive effects at least under basal conditions. It is suggested that the combination of both strategies, although not additive, results into improved cardiac function. © 2013 Elsevier Ireland Ltd © 2013 Published by Elsevier Ireland Ltd.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9