Pex11 alpha in brown trout (Salmo trutta f. fario): Expression dynamics during the reproductive cycle reveals sex-specific seasonal patterns

• Type: Article in International Scientific Journal
• Date: 2013

Authors

castro, lfc (Author) Other; lobo-da-cunha, a (Author) FEUP; rocha, mj (Author) ICBAS; urbatzka, r (Author) Other; rocha, e (Author) FCUP

Journal

Title: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology

Vol. 164

Pages: 207-214

ISSN: 1095-6433

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 5 Citations

Scopus - 4 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-002-22R

DOI: 10.1016/j.cbpa.2012.09.003

Abstract (EN): A negative correlation between female gonadal maturation kinetics and size variations of hepatic peroxisomes was earlier documented in brown trout, as a probable impact of serum estrogen changes during the reproductive cycle. Herein, we investigated whether the organelle volume/surface dynamics seen in female brown trout liver peroxisomes - without numerical changes within each hepatocyte - is followed by variations in the expression of the membrane peroxisome protein Pex11 alpha gene. For comparison, we also studied males. We find in females a seasonal variation with the highest Pex11 alpha expression in February, which was statistically different from all other tested periods. Overall, the expression of PEX11 alpha had over a fivefold decrease from February to September. This period coincides with the reproductive transition between the earlier post-spawning gonadal remodeling and preparatory staging and the pre-spawning period. Males did not show changes. Our approach allowed the first characterization of a peroxin gene in a teleost, the Pex11 alpha, while offering a correlation scenario were, as we hypothesized, the peroxisomal size kinetics is paralleled by membrane-related gene alterations (measured herein as proxy of Pex11 alpha gene expression). Our data support and expand previous results on the regulation, function and morphology of peroxisome dynamics in brown trout, with a broader interest.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8