Incubation temperature induces changes in muscle cellularity and gene expression in Senegalese sole (Solea senegalensis)

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Catarina Campos (Author) Other; Luisa M P Valente (Author) ICBAS; Luis E C Conceicao (Author) Other; Sofia Engrola (Author) Other; Vera Sousa (Author) Other; Eduardo Rocha (Author) ICBAS; Jorge M O Fernandes (Author) Other

Journal

Title: Gene

Vol. 516

Pages: 209-217

ISSN: 0378-1119

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 31 Citations

Scopus - 48 Citations

Scientific classification

FOS: Natural sciences > Earth and related Environmental sciences

Other information

Authenticus ID: P-002-01B

DOI: 10.1016/j.gene.2012.12.074

Abstract (EN): Fertilised eggs of Senegalese sole were incubated at 15, 18 or 21 degrees C, and after hatching all larvae were reared at 21 degrees C until 30 days post-hatch. By this point larvae from the 18 or 21 degrees C temperature groups had 11 and 9% more muscle fibres than those from 15 degrees C, respectively. Hyperplastic growth during metamorphosis was higher in larvae from 18 degrees C. Embryonic temperature induced gene expression changes, albeit with a variable pattern throughout development Myf5, myod2, myHC and fst mRNA levels were significantly higher at several stages prior to hatching in embryos incubated at 21 degrees C, whereas hsp90AB and hsp70 transcripts were present at higher levels in the 15 degrees C group. Myf5, myod1, myod2, pax7, myog, fst, igf-II, igf1r, hsp90AA and hsp90AB were expressed at higher levels during early development, particularly during somitogenesis. In contrast, mrf4, myHC, mylc2, igf-I, mstn1 and hsp70 were up-regulated at later stages of larval development, namely during and after metamorphosis. This study is the first example of thermal plasticity of myogenesis with prolonged effect in a flatfish.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9