Use of destained cytology slides for the application of routine special stains

• Type: Article in International Scientific Journal
• Date: 2009

Authors

marcos, r (Author) Other; santos, m (Author) Other; santos, n (Author) Other; malhao, f (Author) Other; ferreira, f (Author) Other; monteiro, raf (Author) ICBAS; rocha, e (Author) ICBAS

Journal

Title: Veterinary Clinical Pathology

Vol. 38

Pages: 94-102

ISSN: 0275-6382

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 5 Citations

Scopus - 9 Citations

Scientific classification

FOS: Agrarian Sciences > Veterinary science

Other information

Authenticus ID: P-003-N3J

DOI: 10.1111/j.1939-165x.2008.00098.x

Abstract (EN): Background: Special stains to demonstrate microorganisms or intra- and extracellular substances have not been evaluated in detail regarding their applicability and usefulness in destained cytologic specimens. Objectives: The aim of this study is to compare the results of routine special stains on destained slides previously stained with Hemacolor and on fresh (unstained) specimens. Methods: Archival Hemacolor-stained fine needle aspirate specimens of inflammation with infectious agents ( bacterial, mycobacterial, and fungal infections), neoplasia ( melanoma, myxosarcoma, and mammary adenocarcinoma), and hemorrhage ( pericardial effusion) from 14 dogs and 7 cats were selected. Cells in a minimum of 4 fields were photographed and 5 slides from each case were then destained by different methods ( alcohol acid or microwave). Seven special stains were applied selectively to the destained slides, depending on the cytologic findings: periodic acid Schiff, Grocott-Gomori methenamine silver, Gram's, Ziehl-Neelsen, Alcian blue, Fontana-Masson, and Prussian blue. The same fields were rephotographed and 2 observers evaluated the slides qualitatively, with comparison to fresh cytologic specimens from similar lesions. Results: Special stains applied to destained slides demonstrated the expected cellular and extracellular material or organisms independent of the destaining method. Staining intensity, nonspecific staining ( background), cell morphology, and nuclear counterstaining results were similar to those of special stains applied to fresh unstained slides. Conclusions: Destaining does not appear to affect the results of routine special staining for cytologic specimens. Destaining before special stains may be a valuable diagnostic strategy when few slides are present or only stained slides are available.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9