Comparison between two common methods for measuring Giardia lamblia susceptibility to antiparasitic drugs in vitro

• Type: Article in International Scientific Journal
• Date: 2003

Authors

cruz, a (Author) Other; sousa, mi (Author) Other; azeredo, z (Author) Other; silva, mc (Author) ICBAS; de sousa, jcf (Author) Other; manso, o (Author) Other; cabral, m (Author) Other

Journal

Title: Acta Tropica

Vol. 88

Pages: 131-135

ISSN: 0001-706X

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 3 Citations

Scopus - 5 Citations

Scientific classification

FOS: Medical and Health sciences > Health sciences

Other information

Authenticus ID: P-000-EVN

DOI: 10.1016/S0001-706X(03)00196-7

Abstract (EN): In this study a comparison between two different methods for measuring the susceptibility of Giardia lamblia trophozoites to metronidazole and albendazole is performed. Modifications of Meloni's method, based on the loss of adherence of parasites to surfaces, and the Hill method, based on the loss of parasite division capacity, are compared. A logistic model was used to calculate the inhibitory concentrations IC10, IC50 and IC90 that were further compared using the respective standard errors. The results obtained, after contact of parasites with the antiparasitic drugs for 24 h, show that the adherence method is more sensitive than the multiplication method for low and moderate inhibitory concentrations of albendazole. Conversely for metronidazole the multiplication method seems to be more sensitive for high inhibitory concentrations of the drug. For screening the IC50, both methods seem to be effective; however, the inhibition of adherence method have even better performance for the benzimidazole like drugs. (C) 2003 Elsevier B.V. All rights reserved.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5