Alkaline phosphatase from rat liver and kidney is differentially modulated

• Type: Article in International Scientific Journal
• Date: 2001

Authors

Martins, MJ (Author) FMUP; Negrao, MR (Author) FMDUP; Hipolito Reis, C (Author) Other

Journal

Title: Clinical Biochemistry

Vol. 34

Pages: 463-468

ISSN: 0009-9120

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 35 Citations

Scopus - 45 Citations

Scientific classification

FOS: Engineering and technology > Medical engineering

Other information

Authenticus ID: P-000-TBT

DOI: 10.1016/s0009-9120(01)00255-7

Abstract (EN): Objective: To investigate the effect of inhibitors of alkaline phosphatase (ALP) and modulators of P-glycoprotein (Pgp), multidrug resistance protein (MRP) and hepatic taurocholate uptake on the activity of tissue-nonspecific ALP (TNALP) in Ever and kidney. Design and Results: ALP activity was determined in rat liver and kidney homogenates. Levamisole had a stronger inhibitory effect on renal TNALP than on the hepatic isoform. 1,3-dimethylxanthine (theophylline) almost abolished renal TNALP activity whereas its effect on hepatic TNALP was less intense. 3-isobutyl-1-methylxanthine (EBMX) and lidocaine produced opposite effects, activating hepatic TNALP and inhibiting the kidney isoform. Quinidine significantly inhibited renal TNALP without affecting hepatic TNALP. Kaempferol activated both liver and kidney isoforms, the effect being more pronounced on hepatic TNALP. Conclusions: a) renal TNALP seems to be more sensitive to inhibition than hepatic TNALP, b) TNALP activity studies should take into account the source of ALP isoform and c) ALP pharmacological manipulation in vivo may produce different and even opposite effects in different tissues/organs.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6