IN-VIVO EVIDENCE FOR A NON-T CELL ORIGIN OF INTERLEUKIN-5

• Type: Article in International Scientific Journal
• Date: 1995

Authors

CASTRO, AG (Author) Other; SILVA, RA (Author) Other; MINOPRIO, P (Author) Other; APPELBERG, R (Author) ICBAS

Journal

Title: Scandinavian Journal of Immunology

Vol. 41

Pages: 288-292

ISSN: 0300-9475

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 5 Citations

Scopus - 6 Citations

Scientific classification

FOS: Medical and Health sciences > Basic medicine

Other information

Authenticus ID: P-001-H8Q

DOI: 10.1111/j.1365-3083.1995.tb03566.x

Abstract (EN): Eosinophil myelopoiesis is to a great extent regulated by interleukin (IL)-5. Analysis of IL-5 mRNA in spleen cell preparations by reverse transcription-polymerase chain reaction (RT-PCR) revealed the presence of message for this cytokine in uninfected severe combined immunodeficiency (SCID) mice. This message was increased following Mycobacterium avium infection. Normal BALB/c mice had higher levels of expression of IL-5 but the expression of this cytokine was reduced during M. avium infection. Anti-IL-5 monoclonal antibody administration in vivo to SCID mice reduced the number of peritoneal and splenic eosinophils. Gamma interferon (IFN-gamma) had an inhibitory effect of eosinophilopoiesis during infection of SCID mice by M. avium since neutralization of this cytokine increased the number of eosinophils detected in the peritoneal cavity of infected animals. Our results suggest that IL-5 may be produced by cells other than T cells that are both able to respond to infection and are under the control of IFN-gamma.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5