Unmasking antigen determinants in amyloid

• Type: Article in International Scientific Journal
• Date: 1986

Authors

costa, pp (Author) Other; jacobsson, b (Author) Other; collins, vp (Author) Other; biberfeld, p (Author) Other

Journal

Title: Journal of Histochemistry and Cytochemistry

Pages: 1683-1685

ISSN: 0022-1554

Publisher: Histochemical Society

Indexing

ISI Web of Knowledge

Scopus - 25 Citations

Scientific classification

FOS: Medical and Health sciences > Health sciences

Other information

Authenticus ID: P-008-QAY

Abstract (EN): Rehydrated paraffin sections of formalin-fixed, amyloid-containing tissues were treated with denaturing agents (guanidine and urea) and reducing agents (DDT and mercaptoethanol) before immunostaining, in an attempt to expose antigenic determinants hidden in the rigid structure of amyloid fibrils. Pre-treatment overnight with 6 M guanidine or urea was found beneficial, especially in specimens from familial amyloid polyneuropathy of the Portugese type. Addition of reducing agents had no major effect. Modifications of this method may be useful in unmasking other antigens that are polymerized or considered destroyed by fixation and paraffin embedding.

Language: English

Type (Professor's evaluation): Scientific