PCR amplification of DNA obtained from archived hematoxylin-eosin- and Giemsa-stained breast cancer aspirates

• Type: Article in International Scientific Journal
• Date: 1998

Authors

schmitt, fc (Author) Other; soares, r (Author) FMDUP; cirnes, l (Author) Other; seruca, r (Author) Other

Journal

Title: Diagnostic Cytopathology

Vol. 19

Pages: 395-397

ISSN: 8755-1039

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 11 Citations

Scopus - 11 Citations

Other information

Authenticus ID: P-001-6TB

DOI: 10.1002/(sici)1097-0339(199811)19:5<395::aid-dc19>3.3.co;2-w

Abstract (EN): The study of DNA abnormalities in fine-needle aspiration (FNA) specimens for breast cancer could be helpful in improving the capacity of diagnosis and specially to obtain prognostic or predictive information. The nim of the present study was to verify whether it is possible to perform molecular analysis in slides of breast cancer FNA specimens, previously stained by hematoxylineosin (H&E) and Giemsa, stored at least for 3 years. For this purpose, 10 cases of FNA obtained from breast cancer patients diagnosed between 1993 and 1994 in our institute were used Five cytologic smears were alcohol-fixed and stained with H&E. The other Jive were air-fixed and Giemsa stained. DNA was isolated from the cytologic smears and amplified by using radioactive polymerase chain reaction (PCR) aimed at BAT-26 marker Our results demonstrate that archived stained smears prepared for cytologic examinations can be used for molecular analyses by using a PCR amplification method. DNA could be isolated and PCR amplified independently of the prior fixation and staining procedures. So, we conclude that the application of molecular biology techniques to the existing archival smears may become a valuable tool to detect genetic changes in samples from breast cancer aspirates. Diagn. Cytopathol. 1998;19:395-397. (C) 1998 Wiley-Liss,Inc.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 3