Gilthead seabream (Sparus aurata) immune responses are modulated after feeding with purified antinutrients

• Type: Article in International Scientific Journal
• Date: 2014

Authors

Benjamin Costas (Author) Other; Ana Couto (Author) Other; Rita Azeredo (Author) Other; Marina Machado (Author) Other; Ashild Krogdahl (Author) Other; Aires Oliva Teles (Author) FCUP

Journal

Title: Fish and Shellfish Immunology

Vol. 41

Pages: 70-79

ISSN: 1050-4648

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 25 Citations

Scopus - 27 Citations

Scientific classification

FOS: Agrarian Sciences > Agriculture, Forestry, and Fisheries

Other information

Authenticus ID: P-009-TSK

DOI: 10.1016/j.fsi.2014.05.032

Abstract (EN): The present study aimed to evaluate the effects of two purified antinutrients, soy saponins and phytosterols, in an important species for Mediterranean aquaculture. For this purpose, gilthead seabream (Sparus aurata) were fed six experimental diets containing two levels of those antinutrients, alone or in combination, and a control diet, to apparent visual satiation under controlled conditions. Blood and head-kidney were collected at 7, 15 and 48 days following first feeding in order to assess immune parameters and the expression of immune-related genes. Plasma bactericidal and alternative complement pathway activities increased in fish fed antinutrients compared to fish fed the control diet during the course of the experiment, with more important changes at 7 and 48 days for bactericidal activity and at 7 and 15 days for complement values. In contrast, plasma total immunoglobulins (Ig) increased in fish fed antinutrients only at 48 days. Caspase 1 (casp1), interleukin 18 (1118), colony-stimulating factor-1 receptor (csfr) and hepcidin (hep) presented similar patterns of expression with more important changes at 7 and 48 days, while interleukin 10 (1110) and beta-defensin (def) were mainly up-regulated in fish fed antinutrients at 48 days. The level of expression of 104 increased already at 7 days in fish fed the low concentration of both saponins and phytosterols while a general up-regulation was observed at 48 days compared to fish fed the control diet. Results suggest that feeding seabream a diet with purified saponins and phytosterols, alone or in combination, induces a number of changes that are related to the development of inflammation, with most important changes in fish fed the lower phytosterols concentration.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10