Immunohistochemical localization of angiotensin II receptor types 1 and 2 in the mesenteric artery from spontaneously hypertensive rats

• Type: Article in International Scientific Journal
• Date: 2007

Authors

Carmen Diniz (Author) FFUP; Sandra Leal (Author) Other; Karen Logan (Author) Other; Carolina Rocha Pereira (Author) Other; Ana Sofia Soares (Author) Other; Eduardo Rocha (Author) ICBAS; Jorge Goncalves (Author) FFUP; Paula Fresco (Author) FFUP

Journal

Title: Microscopy Research and Technique

Vol. 70 No. 8

Pages: 677-681

ISSN: 1059-910X

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 6 Citations

Scopus - 6 Citations

Scientific classification

FOS: Medical and Health sciences > Basic medicine

Other information

Authenticus ID: P-004-8KJ

DOI: 10.1002/jemt.20453

Abstract (EN): Angiotensin II plays a crucial role in the control of blood pressure, acting at AT, or AT(2) receptors, and can act as a potent vasoconstrictor of the peripheral vasculature inducing hypertrophy, hyperplasia, or both, in resistance arteries. The aim of the present study was to investigate whether the pattern of distribution of angiotensin AT, and AT2 receptors on mesenteric artery sections differs in spontaneously hypertensive rats (SHR) versus their respective controls (Wistar-Kyoto [WKY] rats). Immunohistochemistry using anti-AT, or anti-AT(2) antibodies was performed on perfused-fixed/paraffin-embedded mesenteric arteries from SHR and WKY rats. 3,3'-Diaminobenzidine tetrabydrochloride (DAB; activated by hydrogen peroxide) staining revealed distinct AT, and AT(2) labeling of all artery layers (adventitia, media and intima) from WK`Y rats, whereas in SHR an abundant AT, labeling was found in both intima and adventitia and a sparser labeling in the media. There was a vast reduction of AT(2) labeling throughout all layers. These results suggest a crucial role for AT(2) receptors in the pathogenesis of hypertension.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5