Nanoliposomes for encapsulation and delivery of the potential antitumoral methyl 6-methoxy-3-(4-methoxyphenyl)-1H-indole-2-carboxylate

• Type: Article in International Scientific Journal
• Date: 2011

Authors

Ana S Abreu (Author) Other; Elisabete M S Castanheira (Author) Other; Maria Joao R P Queiroz (Author) Other; Paula M T Ferreira (Author) Other; Luis A Vale Silva (Author) Other; Eugenia Pinto (Author) FFUP

Journal

Title: Nanoscale Research Letters

Vol. 6

Pages: 1-6

ISSN: 1931-7573

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 46 Citations

Scopus - 56 Citations

Scientific classification

FOS: Engineering and technology > Materials engineering

Other information

Authenticus ID: P-002-NT6

DOI: 10.1186/1556-276x-6-482

Abstract (EN): A potential antitumoral fluorescent indole derivative, methyl 6-methoxy-3-(4-methoxyphenyl)-1H-indole-2carboxylate, was evaluated for the in vitro cell growth inhibition on three human tumor cell lines, MCF-7 (breast adenocarcinoma), A375-C5 (melanoma), and NCI-H460 (non-small cell lung cancer), after a continuous exposure of 48 h, exhibiting very low GI(50) values for all the cell lines tested (0.25 to 0.33 mu M). This compound was encapsulated in different nanosized liposome formulations, containing egg lecithin (Egg-PC), dipalmitoyl phosphatidylcholine (DPPC), dipalmitoyl phosphatidylglycerol (DPPG), DSPC, cholesterol, dihexadecyl phosphate, and DSPE-PEG. Dynamic light scattering measurements showed that nanoliposomes with the encapsulated compound are generally monodisperse and with hydrodynamic diameters lower than 120 nm, good stability and zeta potential values lower than -18 mV. Dialysis experiments allowed to monitor compound diffusion through the lipid membrane, from DPPC/DPPG donor liposomes to NBD-labelled lipid/DPPC/DPPG acceptor liposomes.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6