Insertion of Pex5p into the peroxisomal membrane is cargo protein-dependent

• Type: Article in International Scientific Journal
• Date: 2003

Authors

gouveia, am (Author) FFUP; guimaraes, cp (Author) Other; oliveira, me (Author) Other; sa-miranda, c (Author) Other; azevedo, je (Author) ICBAS

Journal

Title: Journal of Biological Chemistry

Vol. 278

Pages: 4389-4392

ISSN: 0021-9258

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 76 Citations

Scopus - 77 Citations

Other information

Authenticus ID: P-000-HRN

DOI: 10.1074/jbc.c200650200

Abstract (EN): It is now generally accepted that Pex5p, the receptor for most peroxisomal matrix proteins, cycles between the cytosol and the peroxisomal compartment. According to current models of peroxisomal biogenesis, this intracellular trafficking of Pex5p is coupled to the transport of newly synthesized peroxisomal proteins into the organelle matrix. However; direct evidence supporting this hypothesis was never provided. Here, using an in vitro peroxisomal import system, we show that insertion of Pex5p into the peroxisomal membrane requires the presence of cargo proteins. Strikingly the peroxisomal docking translocation machinery is also able to catalyze the membrane insertion of a Pex5p truncated molecule lacking any known cargo-binding domain. These results suggest that the cytosol/peroxisomal cycle in which Pex5p is involved is directly or indirectly regulated by Pex5p itself and not by the peroxisomal docking/translocation machinery.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 4