The energetics of Pex5p-mediated peroxisomal protein import

• Type: Article in International Scientific Journal
• Date: 2003

Authors

oliveira, me (Author) Other; gouveia, am (Author) FFUP; pinto, ra (Author) Other; sa-miranda, c (Author) Other; azevedo, je (Author) ICBAS

Journal

Title: Journal of Biological Chemistry

Vol. 278

Pages: 39483-39488

ISSN: 0021-9258

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 75 Citations

Scopus - 80 Citations

Other information

Authenticus ID: P-000-ETV

DOI: 10.1074/jbc.m305089200

Abstract (EN): Most newly synthesized peroxisomal matrix proteins are targeted to the organelle by Pex5p, the peroxisomal cycling receptor. According to current models of peroxisomal biogenesis, Pex5p interacts with cargo proteins in the cytosol and transports them to the peroxisomal membrane. After delivering the passenger protein into the peroxisomal matrix, Pex5p returns to the cytosol to catalyze additional rounds of transportation. Obviously, such cyclic pathway must require energy, and indeed, data confirming this need are already available. However, the exact step(s) of this cycle where energy input is necessary remains unclear. Here, we present data suggesting that insertion of Pex5p into the peroxisomal membrane does not require ATP hydrolysis. This observation raises the possibility that at the peroxisomal membrane ATP is needed predominantly (if not exclusively) downstream of the protein translocation step to reset the Pex5p-mediated transport system.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6