Exo- and endo-glucanolytic activity of cellulases purified from Trichoderma reesei

• Type: Article in International Scientific Journal
• Date: 1998

Authors

Gama, FM (Author) Other; Vilanova, M (Author) ICBAS; Mota, M (Author) Other

Journal

Title: Biotechnology Techniques

Vol. 12 No. 9

Pages: 677-681

ISSN: 0951-208X

Publisher: Kluwer Academic Publishers

Indexing

ISI Web of Knowledge - 6 Citations

Scopus - 10 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-001-74H

DOI: 10.1023/a:1008808803109

Abstract (EN): Four cellulases, produced by Trichoderma reesei, have been purified by preparative isoelectric focusing (Rotofor), size exclusion (Sephacryl 100 HR), anionic (Mono Q) and cationic (Mono S) chromatography and chromatofocusing (Mono P). Enzymatic activity with a large number of substrates allowed the proteins to be classified as: cellobiohydrolase I, cellobiohydrolase II, endoglucanase I and endoglucanase II. The exo- or endo-glucanase character of these enzymes was analysed by using a technique based on the measurement of the Avicel insoluble fibres reducing power.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5