Unraveling the Uptake Mechanisms of Mannan Nanogel in Bone-Marrow-Derived Macrophages

• Type: Article in International Scientific Journal
• Date: 2012

Authors

Silvia A Ferreira (Author) Other; Alexandra Correia (Author) ICBAS; Pedro Madureira (Author) Other; Manuel Vilanova (Author) ICBAS; Francisco M Gama (Author) Other

Journal

Title: Macromolecular Bioscience

Vol. 12

Pages: 1172-1180

ISSN: 1616-5187

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 6 Citations

Scopus - 6 Citations

Scientific classification

FOS: Engineering and technology > Materials engineering

CORDIS: Technological sciences > Technology > Biotecnology

Other information

Authenticus ID: P-002-62F

DOI: 10.1002/mabi.201200075

Abstract (EN): The mechanisms associated with the cellular internalization of nanomedicines must be carefully considered when designing drug- and vaccine-delivery systems. The cellular fate and effects of nanomedicines depend to a large extent on the cell uptake routes. A self-assembled mannan nanogel is developed as a vaccination platform for antigen and adjuvant delivery. The mannan nanogel uptake by murine bone-marrow-derived macrophages is found to be time-, concentration-, and energy-dependent, involving mannose-receptor-mediated phagocytosis and clathrin-mediated endocytosis. The nanogel is also visualized in the cytosol suggesting endolysosomal escape. These results indicate that mannan nanogel is a promising versatile carrier for intracellular delivery of vaccines or therapeutic agents.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9