Characterisation of the last Fe-S cluster-binding subunit of Neurospora crassa complex I

• Type: Article in International Scientific Journal
• Date: 1999

Authors

sousa, r (Author) Other; barquera, b (Author) Other; duarte, m (Author) Other; finel, m (Author) Other

Journal

Title: Biochimica et Biophysica Acta - Bioenergetics

Vol. 1411

Pages: 142-146

ISSN: 0005-2728

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 7 Citations

Scopus - 7 Citations

Other information

Authenticus ID: P-001-4K5

DOI: 10.1016/S0005-2728(99)00014-6

Abstract (EN): We have cloned cDNAs encoding the last iron-sulphur protein of complex I from Neurospora crassa. The cDNA sequence contains an open reading frame that codes for a precursor polypeptide of 226 amino acid residues with a molecular mass of 24 972 Da. Our results indicate that the mature protein belongs probably to the peripheral arm of complex I and is rather unstable when not assembled into the enzyme. The protein is highly homologous to the PSST subunit of bovine complex I, the most likely candidate to bind iron-sulphur cluster N-2. All the amino acid residues proposed to bind such a cluster are conserved in the fungal protein. (C) 1999 Elsevier Science B.V. All rights reserved.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5