Composition of complex I from Neurospora crassa and disruption of two "accessory" subunits

• Type: Article in International Scientific Journal
• Date: 2005

Authors

marques, i (Author) Other; duarte, m (Author) ICBAS; assuncao, j (Author) Other; ushakova, av (Author) Other

Journal

Title: Biochimica et Biophysica Acta - Bioenergetics

Vol. 1707

Pages: 211-220

ISSN: 0005-2728

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 35 Citations

Scopus - 37 Citations

Other information

Authenticus ID: P-000-3XJ

DOI: 10.1016/j.bbabio.2004.12.003

Abstract (EN): Respiratory chain complex I of the fungus Neurospora crassa contains at least 39 polypeptide subunits, of which 35 are conserved in mammals. The 11.5 kDa and 14 kDa proteins, homologues of bovine IP15 and B16.6, respectively, are conserved among eukaryotes and belong to the membrane domain of the fungal enzyme. The corresponding genes were separately inactivated by repeat-induced point-mutations, and null-mutant strains of the fungus were isolated. The lack of either subunit leads to the accumulation of distinct intermediates of the membrane arm of complex I. In addition, the peripheral arm of the enzyme seems to be formed in mutant nuo 14 but, interestingly, not in mutant nuo 11.5. These results and the analysis of enzymatic activities of mutant mitochondria indicate that both polypeptides are required for complex I assembly and function. © 2004 Elsevier B.V. All rights reserved.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10