Recovery of Peniophora cinerea laccase using aqueous two-phase systems composed by ethylene oxide/propylene oxide copolymer and potassium phosphate salts

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Sérgio Moreira (Author) Other; Sara C. Silvério (Author) FEUP; Eugénia A. Macedo (Author) FEUP; Adriane M. F. Milagres (Author) Other; José A. Teixeira (Author) Other; Solange I. Mussatto (Author) Other

Journal

Title: Journal of Chromatography A

Vol. 1321

Pages: 14-20

ISSN: 0021-9673

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 16 Citations

ISI Web of Science

Scopus - 23 Citations

Scientific classification

FOS: Engineering and technology

CORDIS: Technological sciences

Associated Projects

LA 20 - PROJECTO ESTRATÉGICO - LA 20 - 2013-2014

Other information

Authenticus ID: P-006-J3Y

DOI: 10.1016/j.chroma.2013.10.056

Abstract (EN): Aqueous two-phase systems (ATPSs) composed by UCON (ethylene oxide/propylene oxide copolymer) and potassium phosphate salts were for the first time evaluated in the recovery of Peniophora cinerea laccase from complex fermented medium. The ATPSs were obtained by combining the random copolymer UCON with KH2PO4, potassium phosphate buffer pH 7 or K2HPO4. According to the results, protein partition occurred predominantly toward the saline phase (bottom phase) of the ATPSs, while some contaminants such as pigments partitioned mainly to the top phase. In preliminary tests, it was found that the salt with the lowest pH value (KH2PO4 , pH 4.6) stimulated the enzyme activity, while the other salts (pH between 7.0 and 9.5) caused a strong inhibition. However, the salt inhibition was not observed in the equilibrium phases of the UCON-Potassium phosphate ATPSs. The laccase recovery was high for all the biphasic systems, but the highest value (134%) was obtained when using UCON combined with KH2PO4. When compared to conventional concentration and purification methods (Iyophilization, ammonium sulfate precipitation, ultrafiltration, and ion exchange chromatography), ATPS was demonstrated to be an efficient alternative for P. cinerea laccase recovery from fermented medium.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7