Electrochemical sensing of silver tags labelled DNA immobilized onto disposable graphite electrodes

• Type: Article in International Scientific Journal
• Date: 2007

Authors

Hakan Karadeniz (Author) Other; Arzum Erdem (Author) Other; Ayfer Caliskan (Author) Other; Carlos M Pereira (Author) FCUP; Elisa M Pereira (Author) Other; Jose A Ribeiro (Author) Other

Journal

Title: Electrochemistry Communications

Vol. 9

Pages: 2167-2173

ISSN: 1388-2481

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 53 Citations

Scopus - 52 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-004-7Q6

DOI: 10.1016/j.elecom.2007.05.016

Abstract (EN): An electrochemical approach for the improved electrochemical sensing of DNA was developed in this study based on the oxidation signals of silver and DNA base, guanine by using disposable pencil graphite electrode (PGE) electrodes. The easy surface modification of disposable electrodes PGEs with nucleic acids was performed by passive adsorption using amino linked DNA oligonucleotide attached onto the surface of silver nanoparticles (Ag-NPs). Firstly, the microscopic characterization of silver nanoparticles was investigated by transmission electron microscopy (TEM) and scanning electron microscopy (SEM), and the electrochemical behaviour of these NPs was studied by using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques. Then, the overall performance of this novel electrochemical DNA sensing method based on these nanoparticles is studied and discussed in terms of optimum analytical conditions, such as; the effect of DNA concentration, NPs concentration and different buffer solutions, etc. in order to obtain silver and guanine oxidation signals in higher sensitivity and selectivity. The main features related with this electrochemical assay based on silver nanoparticles are discussed and compared with other assays reported in the literatures.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7