Amperometric glucose biosensor based on assisted ion transfer through gel-supported microinterfaces

• Type: Article in International Scientific Journal
• Date: 2004

Authors

Pereira, CM (Author) FCUP; Oliveira, JM (Author) Other; Silva, RM (Author) Other; Silva, F (Author) FCUP

Journal

Title: Analytical Chemistry

Vol. 76

Pages: 5547-5551

ISSN: 0003-2700

Publisher: American Chemical Society

Indexing

ISI Web of Knowledge - 39 Citations

Scopus - 40 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-000-8HK

DOI: 10.1021/ac0498765

Abstract (EN): A novel amperometric glucose sensor was developed based on the facilitated proton transfer across microinterfaces between two immiscible electrolyte solutions. The combination of a 1,3:2,4-dibenzylidene sorbitol/2-nitrophenyl octyl ether gel membrane and 3-(2-pyridyl)-5,6diphenyl-1,2,4-triazine as the ionophore allows the transfer of protons from water to the gellified organic phase; the gel membrane is supported on arrays of microholes drilled on a polyester film. The protons are generated as the result of the dissociation of gluconic acid produced during the enzymatic degradation of glucose by glucose oxidase. The characteristics of the glucose sensor were investigated using several experimental conditions, namely, the concentration of ligand and enzyme. The electrochemical response is typical of an enzymatic electrode and displays a linear behavior in the range 0.2-3 mM glucose. The effect of the experimental parameters of the voltammetric technique was also optimized with the aimof improving sensor sensitivity.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5