Determination of Aldoses, Deoxy-aldoses and Uronic Acids Content in a Pectin-Rich Extract by RP-HPLC-FLD after p-AMBA Derivatization

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Ana S Silva (Author) Other; Valente, IM (Author) Other; Nunes, C (Author) Other; Coimbra, MA (Author) Other; Luis F Guido (Author) FCUP

Journal

Title: Chromatographia

Vol. 76

Pages: 1117-1124

ISSN: 0009-5893

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 4 Citations

Scopus - 7 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-006-7V9

DOI: 10.1007/s10337-013-2510-y

Abstract (EN): A reversed-phase high-performance liquid chromatographic method with fluorometric detection is proposed for the simultaneous determination of different classes of neutral sugars, such as hexoses (galactose, glucose and mannose), pentoses (arabinose and xylose), deoxy-hexoses (fucose and rhamnose), as well as acidic sugars (galacturonic and glucuronic acids). The separation is carried out on a hydrophilic end capped C-18 column following a pre-column derivatization with p-aminobenzoic acid. The fluorometric detection of the derivatives has shown a strong dependency with the mobile phase pH. The performance of the proposed methodology was evaluated and the prerequisites of linearity (r-value > 0.999), precision (intra-day CV < 6 % and inter-day CV < 11 %) and recovery (between 77 +/- A 7 and 103 +/- A 3 %) were satisfied. To our knowledge, the obtained values of limit of detection for neutral sugars (within the range 6.1-28 mu g L-1) are the lowest reported using this derivatizing agent. In order to better judge the methodology presented herein, neutral sugars of a pectin-rich orange extract were also analysed by the conventionally used GC-FID (gas-chromatography with flame ionization detector) method of alditol acetate derivatives. A statistical test (paired t test) has proved that no significant differences (alpha = 0.05) were observed between these two methods.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8