Characterization of monomeric and oligomeric flavan-3-ols from barley and malt by liquid chromatography-ultraviolet detection-electrospray ionization mass spectrometry

• Type: Article in International Scientific Journal
• Date: 2008

Authors

Marketa Dvorakova (Author) Other; Manuela M Moreira (Author) Other; Pavel Dostalek (Author) Other; Zuzana Skulilova (Author) Other; Luis F Guido (Author) FCUP; Aquiles A Barros (Author) FCUP

Journal

Title: Journal of Chromatography A

Vol. 1189

Pages: 398-405

ISSN: 0021-9673

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 56 Citations

Scopus - 62 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-003-ZF7

DOI: 10.1016/j.chroma.2007.10.080

Abstract (EN): The pattern of the monomeric and oligomeric flavan-3-ols for 10 barley varieties and the corresponding malts were identified and quantified using high-performance liquid chromatography-ultraviolet detection-electrospray ion trap mass spectrometry. The Folin-Ciocalteau and the vanillin spectrophotometric assays were used for the assessment of the total polyphenol and total flavan-3-ol content, respectively, and the antioxidant activity was determined as the 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and the ferricyanide reducing power. Catechin and prodelphinidin B3 were respectively the major monomeric and dimeric flavan-3-ols. Moreover, prodelphinidin B3 was shown to be the main contributor for the radical scavenging activity both for barley and malt.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8