The non-protein amino acid beta-N-methylamino-L-alanine in Portuguese cyanobacterial isolates

• Type: Article in International Scientific Journal
• Date: 2012

Authors

Rosa C C Cervantes Cianca (Author) Other; Mafalda S Baptista (Author) FCUP; Viviana R Lopes (Author) Other; Vitor M Vasconcelos (Author) FCUP

Journal

Title: Amino Acids

Vol. 42

Pages: 2473-2479

ISSN: 0939-4451

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 27 Citations

Scopus - 38 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-002-9M1

DOI: 10.1007/s00726-011-1057-1

Abstract (EN): The tailor made amino acid beta-N-methyl-amino-L-alanine (BMAA) is a neurotoxin produced by cyanobacteria. It has been associated with certain forms of progressive neurodegenerative disease, including sporadic Amyotrophic Lateral Sclerosis and Alzheimer's disease. Some different reports of BMAA in cyanobacterial blooms from lakes, reservoirs, and other water resources have been made by different investigators. We here report the detection of BMAA of both free and protein-bound produced by cyanobacteria, belonging to the Chroococcales, Oscillatoriales and Nostocales ordered. We use a rapid and sensitive HPLC-FD method that utilizes methanol elution and the Waters AQC Tag chemistry. On other hand, we have used three different assay procedures for BMAA extraction from cyanobacteria: Trichloroacetic acid (TCA), Methanol/Acetone and hydrochloric acid (HCl). All assays let successfully detect BMAA in all cyanobacteria samples analyzed. Nevertheless, with TCA and HCl extraction procedures the highest BMAA values, for free as well as protein-bound BMAA were detected. BMAA content could not be related to the taxonomy of the isolates or to their geographical origin, and no correlation between free and protein-bound BMAA concentrations were observed within or between taxonomic groups. These data offer confirmation of the taxonomic and geographic ubiquity of BMAA from naturally occurring populations of cyanobacteria, for the first time reported for estuaries.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7