Determination of the non protein amino acid beta-N-methylamino-L-alanine in estuarine cyanobacteria by capillary electrophoresis

• Type: Article in International Scientific Journal
• Date: 2011

Authors

Mafalda S Baptista (Author) FCUP; Rosa C C Cianca (Author) Other; Viviana R Lopes (Author) Other; Marisa M R Almeida (Author) FCUP; Vitor M Vasconcelos (Author) FCUP

Journal

Title: Toxicon

Vol. 58

Pages: 410-414

ISSN: 0041-0101

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 20 Citations

Scopus - 23 Citations

Scientific classification

FOS: Medical and Health sciences > Basic medicine

Other information

Authenticus ID: P-002-KTX

DOI: 10.1016/j.toxicon.2011.08.007

Abstract (EN): A capillary electrophoretic method for the determination of the amino acid beta-N-methylamino-L-alanine (BMAA) was achieved using a fused-silica capillary column (50 cm x 75 pm I.D.) filled with 5 mM sodium tetraborate solution (pH 9), with an applied voltage of 25 kV, at 25 degrees C. The method was then applied in quantifying BMAA in eighteen strains of lyophilized estuarine cyanobacteria, following amino acid extraction using 0.1 M trichloroacetic acid and 6 M hydrochloric acid, sequentially.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5