Experimental and Modeling Analysis of Synechocystis sp PCC 6803 Growth

• Type: Article in International Scientific Journal
• Date: 2012

Authors

Miguel Lopo (Author) Other; Arnau Montagud (Author) Other; Emilio Navarro (Author) Other; Isabel Cunha (Author) Other; Andrea Zille (Author) Other; Pedro F Fernandez de Cordoba (Author) Other; Pedro Moradas Ferreira (Author) ICBAS; Paula Tamagnini (Author) FEUP; Javier F Urchueguia (Author) Other

Journal

Title: Journal of Molecular Microbiology and Biotechnology

Vol. 22

Pages: 71-82

ISSN: 1464-1801

Publisher: S. Karger AG

Indexing

ISI Web of Knowledge - 15 Citations

Scopus - 18 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-002-G1X

DOI: 10.1159/000336850

Abstract (EN): Background/Aims: The influence of different parameters such as temperature, irradiance, nitrate concentration, pH, and an external carbon source on Synechocystis PCC 6803 growth was evaluated. Methods: 4.5-ml cuvettes containing 2 ml of culture, a high-throughput system equivalent to batch cultures, were used with gas exchange ensured by the use of a Parafilm (TM) cover. The effect of the different variables on maximum growth was assessed by a multi-way statistical analysis. Results: Temperature and pH were identified as the key factors. It was observed that Synechocystis cells have a strong influence on the external pH. The optimal growth temperature was 33 degrees C while light-saturating conditions were reached at 40 mu E.m(-2.)s(-1). Conclusion: It was demonstrated that Synechocystis exhibits a marked difference in behavior between autotrophic and glucose-based mixotrophic conditions, and that nitrate concentrations did not have a significant influence, probably due to endogenous nitrogen reserves. Furthermore, a dynamic metabolic model of Synechocystis photosynthesis was developed to gain insights on the underlying mechanism enabling this cyanobacterium to control the levels of external pH. The model showed a coupled effect between the increase of the pH and ATP production which in turn allows a higher carbon fixation rate. Copyright (C) 2012 S. Karger AG, Basel

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 12