HEPATOPROTECTIVE ACTIVITY OF XANTHONES AND XANTHONOLIGNOIDS AGAINST TERT-BUTYLHYDROPEROXIDE-INDUCED TOXICITY IN ISOLATED RAT HEPATOCYTES - COMPARISON WITH SILYBIN

• Type: Article in International Scientific Journal
• Date: 1995

Authors

FERNANDES, ER (Author) Other; CARVALHO, FD (Author) FFUP; REMIAO, FG (Author) FFUP; BASTOS, ML (Author) FFUP; PINTO, MM (Author) FFUP; GOTTLIEB, OR (Author) Other

Journal

Title: Pharmaceutical Research

Vol. 12

Pages: 1756-1760

ISSN: 0724-8741

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 45 Citations

Scopus - 51 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-001-G3P

DOI: 10.1023/a:1016230125496

Abstract (EN): Purpose. Synthesize and evaluate the protective activity against tert-butylhydroperoxide-induced toxicity in freshly isolated rat hepatocytes of trans-kielcorin, trans-isokielcorin B, as well as their respective building blocks 3,4-dihydroxy-2-methoxyxanthone and 2,3-dihydroxy-4-methoxyxanthone. Methods. Wistar rats, weighing 200-2508 were used. Hepatocyte isolation was performed by collagenase perfusion. Incubations were performed at 37 degrees C, using 1 million cells per milliliter in modified Krebs-Henseleit buffer. The protective activity was evaluated by measuring reduced and oxidized glutathione, lipid peroxidation and cell viability after inducing toxicity with tert-butylhydroperoxide (1.0 mM, 30 min), with or without the studied compounds in the concentrations of 0.025, 0.050, 0.100 and 0.200 mM. Silybin was tested in the same experimental conditions to serve as a positive control. Results. Using these concentrations, the tested compounds prevented tert-butylhydroperoxide-induced lipid peroxidation and cell death in freshly isolated rat hepatocytes. All compounds were also effective in preventing perturbation of cell glutathione homeostasis in some extent. 3,4-Dihydroxy-2-methoxyxanthone and 2,3-dihydroxy-4-methoxyxanthone were more effective than trans-kielcorin and trans-isokielcorin B respectively. Silybin was less effective in protecting cells against lipid peroxidation and loss of cell viability than the four xanthonic derivatives. Conclusions. The tested compounds protected the freshly isolated rat hepatocytes against tert-butylhydroperoxide-induced toxicity.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5