Protective effect of Castanea sativa and Quercus robur leaf extracts against oxygen and nitrogen reactive species

• Type: Article in International Scientific Journal
• Date: 2008

Authors

Isabel F Almeida (Author) FFUP; Eduarda Fernandes (Author) FFUP; Jose L F C Lima (Author) FFUP; Costa, PC (Author) FFUP; Bahia, MF (Author) Other

Journal

Title: Journal of Photochemistry and Photobiology B: Biology

Vol. 91 No. 2

Pages: 87-95

ISSN: 1011-1344

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 54 Citations

Scopus - 57 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-003-ZB7

DOI: 10.1016/j.jphotobiol.2008.02.001

Abstract (EN): Topical natural antioxidants are a useful strategy for the prevention of photoaging and oxidative stress mediated skin diseases. In view of this underlying principle, the screening of natural plant extracts with scavenging activity for pro-oxidant reactive species is a primary requirement for the development of new topical antioxidant formulations. In the present study, an ethanol:water (7:3) extract from Castanea sativa leaves and a ethanol:water (2:3) extract from Quercus robur leaves were evaluated for their putative in vitro scavenging effects on reactive oxygen species (ROS) namely superoxide radical (O-2(center dot-)), hydroxyl radical (HO center dot), peroxyl radical (ROO center dot), hydrogen peroxide (H2O2) and singlet oxygen (O-1(2)) as well as on reactive nitrogen species (RNS) namely nitric oxide ((NO)-N-center dot) and peroxynitrite (ONOO-). The extracts presented a high potency to scavenge the tested reactive species, all the IC(50)s being found at the mu g/mL level. IC(50)s (mean +/- SE) for the ROS O-2(center dot-), HO center dot, H2O2 and O-1(2) were 13.6 +/- 1.8; 216 +/- 4; 410 +/- 8; 12.3 +/- 0.7 mu g/mL, respectively, for C sativa, and 11.0 +/- 0.5; 285 +/- 22; 251 +/- 32; 7.90 +/- 0.56 mu g/mL, respectively, for Q. robur. The ORAC values obtained for ROO center dot were 1.24 +/- 0.13 for C sativa and 1.09 +/- 0.06 for Q. robur. The IC(50)s (mean +/- SE) for (NO)-N-center dot and ONOO- were 3.10 +/- 0.14 and 1.49 +/- 0.10 mu g/mL, respectively, for C. sativa and 3.13 +/- 0.11 and 0.95 +/- 0.02 mu g/mL, respectively, for Q. robur. The content of total phenolics for C sativa and Q. robur were 284 +/- 9 and 346 +/- 4 mg of gallic acid equivalents (GAE)/g of lyophilized extract respectively. The observed effects might be of relevance considering the putative interest of these extracts as topical antioxidants.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9