Protective activity of Hypericum androsaemum infusion against tert-butyl hydroperoxide-induced oxidative damage in isolated rat hepatocytes

• Type: Article in International Scientific Journal
• Date: 2004

Authors

Valentao, P (Author) FFUP; Carvalho, M (Author) Other; Fernandes, E (Author) FFUP; Carvalho, F (Author) FFUP; Andrade, PB (Author) FFUP; Seabra, RM (Author) Other; Bastos, MD (Author) FFUP

Journal

Title: Journal of Ethnopharmacology

Vol. 92

Pages: 79-84

ISSN: 0378-8741

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 16 Citations

Scopus - 18 Citations

Scientific classification

FOS: Medical and Health sciences > Basic medicine

Other information

Authenticus ID: P-000-AK3

DOI: 10.1016/j.jep.2004.02.004

Abstract (EN): Hypericum androsaemum L. (Guttiferae) is a medicinal plant with antioxidant activity. Increasing evidence regarding free radical generating agents suggests that hepatotoxic-related disorders may involve reactive oxygen species (ROS). The purpose of this study was to investigate the protective effect of Hypericum androsaemum infusion on isolated rat hepatocytes oxidative injury induced by tert-butyl hydroperoxide (t-BHP). The results showed that pretreatment of the cells with this infusion (16, 62 and 250 mug/ml) prevented the leakage of lactate dehydrogenase (LDH) and lipid peroxidation caused by a 30-min treatment with t-BHP (1 mM). However, infusion-induced alterations on glutathione homeostasis were noticed, as it was observed by the increase in glutathione oxidised form (GSSG) and depletion in total glutathione levels, which indicates that plant-derived antioxidant extracts may not be considered a generalised way of treating pro-oxidant-related diseases. sigma 2004 Elsevier Ireland Ltd. All rights reserved.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6