Cell Therapy with Human MSCs Isolated from the Umbilical Cord Wharton Jelly Associated to a PVA Membrane in the Treatment of Chronic Skin Wounds

• Type: Article in International Scientific Journal
• Date: 2014

Authors

Jorge Ribeiro (Author) Other; Tiago Pereira (Author) Other; Irina Amorim (Author) Other; Ana Rita Caseiro (Author) Other; Lopes, MA (Author) FEUP; Joana Lima (Author) Other; Andrea Gartner (Author) Other; Jose Domingos Santos (Author) FEUP; Paulo J Bartolo (Author) Other; Jorge Manuel Rodrigues (Author) Other; Ana Colette Mauricio (Author) Other; Ana Lucia Luis (Author) Other

Journal

Title: International Journal of Medical Sciences

Vol. 11 No. 10

Pages: 979-987

ISSN: 1449-1907

Publisher: Ivyspring International Publisher

Indexing

ISI Web of Knowledge - 32 Citations

Scopus - 45 Citations

Pubmed / Medline

Scientific classification

FOS: Medical and Health sciences > Clinical medicine

CORDIS: Technological sciences > Engineering > Biomaterial engineering

Other information

Authenticus ID: P-009-SXH

DOI: 10.7150/ijms.9139

Abstract (EN): The healing process of the skin is a dynamic procedure mediated through a complex feedback of growth factors secreted by a variety of cells types. Despite the most recent advances in wound healing management and surgical procedures, these techniques still fail up to 50%, so cellular therapies involving mesenchymal stem cells (MSCs) are nowadays a promising treatment of skin ulcers which are a cause of high morbidity. The MSCs modulate the inflammatory local response and induce cell replacing, by a paracrine mode of action, being an important cell therapy for the impaired wound healing. The local application of human MSCs (hMSCs) isolated from the umbilical cord Wharton's jelly together with a poly(vinyl alcohol) hydrogel (PVA) membrane, was tested to promote wound healing in two dogs that were referred for clinical examination at UPVET Hospital, showing non-healing large skin lesions by the standard treatments. The wounds were infiltrated with 1000 cells/mu l hMSCs in a total volume of 100 mu l per cm(2) of lesion area. A PVA membrane was applied to completely cover the wound to prevent its dehydration. Both animals after the treatment demonstrated a significant progress in skin regeneration with decreased extent of ulcerated areas confirmed by histological analysis. The use of Wharton's jelly MSCs associated with a PVA membrane showed promising clinical results for future application in the treatment of chronic wounds in companion animals and humans.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9