In-vivo in situ en-face optical coherence tomography imaging of chick embryos

• Type: Article in International Conference Proceedings Book
• Date: 2009

Authors

Leitner, M (Author) Other; Castanheira, J (Author) Other; Ferreira, L (Author) Other; Ferreira, M (Author) Other; Palmeirim, I (Author) Other; Rosa, CC (Author) FCUP; Podoleanu, AG (Author) Other

Conference proceedings International

Title: Progress in Biomedical Optics and Imaging - Proceedings of SPIE

Optical Coherence Tomography and Coherence Techniques IV

Munich, 14 June 2009 through 17 June 2009

Indexing

ISI Web of Knowledge

Scopus - 2 Citations

Other information

Authenticus ID: P-007-YSX

DOI: 10.1117/12.831859

Abstract (EN): Chick embryos are among the most studied species in development biology because they are easily obtained, highly accessible and present a similar development to that of humans. Normally morphological studies are carried out with confocal microscopy, however in-situ imaging is impossible and in-vivo imaging can only be performed with great difficulty. For confocal microscopy the embryo has to be studied outside the egg, what generally also means a short life expectancy of the embryo. Additionally, extracting the embryo of the egg precludes the possibility of studying its development in its natural environment. In this paper it is shown that en-face optical coherence tomography (en-face OCT) is a possible solution to overcome these difficulties allowing for an in-situ and in-vivo study over a timescale of several days. With en-face OCT it is possible to accompany the development of one single embryo over several days and to acquire high resolution and axially resolved images. © 2009 SPIE-OSA.

Language: English

Type (Professor's evaluation): Scientific