Electrospray tandem mass spectrometry of aminochromes

• Type: Article in International Scientific Journal
• Date: 2001

Authors

Lemos Amado, F (Author) Other; Domingues, P (Author) Other; Ferrer Correia, A (Author) Other; Remiao, F (Author) FFUP; Milhazes, N (Author) Other; Borges, F (Author) FFUP; Carvalho, FD (Author) FFUP; Bastos, ML (Author) FFUP

Journal

Title: Rapid Communications in Mass Spectrometry

Vol. 15

Pages: 2466-2471

ISSN: 0951-4198

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 16 Citations

Scopus - 15 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-000-XV0

DOI: 10.1002/rcm.498

Abstract (EN): The catecholamines adrenaline, noradrenaline, dopamine, dopa and isoprenaline were oxidized into their respective aminochromes: adrenochrome, noradrenochrome, dopaminochrome, dopachrome and isoprenochrome. Tandem mass spectrometry (MS/MS) fragmentation patterns were examined for the five aminochromes in order to establish a general structural assignment of these oxidation products by electrospray mass spectrometry. Although protonated aminochromes undergo similar fragmentation patterns with a characteristic consecutive loss of two carbonyl groups, the presence of different substituents in the parent compounds led to significant changes in the CID spectra. This feature is more evident for isoprenochrome and dopachrome, especially for the latter where the MS/MS spectrum is dominated by the loss of formic acid. A general pattern of fragmentation for aminochromes is proposed, which should provide a suitable basis to aid their characterization in studies in vivo or in vitro. Copyright (C) 2001 John Wiley & Sons, Ltd.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6