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SIMULTANEOUS ISOCRATIC HPLC SEPARATION OF THE DIASTEREOISOMERS OF CAFFEIC, FERULIC, AND ISOFERRULIC ACIDS AND RELATED COUMARINS

Title
SIMULTANEOUS ISOCRATIC HPLC SEPARATION OF THE DIASTEREOISOMERS OF CAFFEIC, FERULIC, AND ISOFERRULIC ACIDS AND RELATED COUMARINS
Type
Article in International Scientific Journal
Year
1993
Authors
BORGES, MFM
(Author)
FFUP
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ROLEIRA, FMF
(Author)
Other
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PINTO, MMM
(Author)
FFUP
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Journal
Vol. 16
ISSN: 0148-3919
Publisher: Marcel Dekker Inc.
Scientific classification
FOS: Natural sciences > Chemical sciences
Other information
Authenticus ID: P-001-NNY
Abstract (EN): A general procedure for the simultaneous analysis of phenolic compounds by a reversed-phase high performance liquid chromatography (RP-HPLC) is described. The series of compounds embraces the cis/trans isomers of caffeic, ferulic and isoferulic acids and the coumarins - esculetin, scopoletin and isoscopoletin. In order to establish the experimental conditions, leading to an optimal resolution, several test mixtures were analysed. HPLC separation was carried out on a reversed-phase column ( Lichrosorb RP-8, 5 mum), using aqueous tetrahydrofuran as a mobile phase.
Language: English
Type (Professor's evaluation): Scientific
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