Procyanidins as antioxidants and tumor cell growth modulators

• Type: Article in International Scientific Journal
• Date: 2006

Authors

Faria, A (Author) FMUP; Calhau, C (Author) FMUP; de Freitas, V (Author) FCUP; Mateus, N (Author) FCUP

Journal

Title: Journal of Agricultural and Food Chemistry

Vol. 54

Pages: 2392-2397

ISSN: 0021-8561

Publisher: American Chemical Society

Indexing

ISI Web of Knowledge - 115 Citations

Scopus - 137 Citations

Scientific classification

FOS: Agrarian Sciences > Other Agrarian Sciences

Other information

Authenticus ID: P-004-MPX

DOI: 10.1021/jf0526487

Abstract (EN): Five procyanidin fractions with different structural complexities were obtained after fractionation of a grape seed extract. The procyanidin fraction's abilities to inhibit lipid peroxidation induced by 2,2'-azobis-2-methyl-propanimidamide dihydrochloride in a liposomal membrane system were examined. The antioxidant capacities of all fractions were evaluated through monitoring oxygen consumption and by measuring the formation of conjugated dienes. All tested fractions provided protection of membranes against peroxyl radicals by increasing the induction time of oxidation. This effect increased up to fraction II but decreased with the increase of the structural complexity of further procyanidin fractions, possibly due to steric hindrance effects exhibited by the more complex fractions. In addition, the antiradical properties and the reducing power of these fractions were determined by using 2,2diphenyl-1-picrylhydrazyl and ferric reducing/antioxiclant power methods, respectively. Moreover, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-bromide reduction and DNA synthesis were measured in Michigan Cancer Foundation 7 (MCF-7), a human breast cancer cell line, treated with catechin or procyanidin fractions in order to evaluate the effect of these compounds on cell viability and proliferation. The results obtained showed that at 30 mu g/mL, fractions I and 11 decreased cell viability and proliferation, which was not observed with 60 mu g/mL of the same fractions. Catechin was also able to decrease cell viability and proliferation at 30 and 60 mu g/mL. It is interesting to notice that the procyaniclin fractions that exhibited higher antioxidant activity were the same to affect cell viability and proliferation.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6