Interaction of rifampicin and isoniazid with large unilamellar liposomes: spectroscopic location studies

• Type: Article in International Scientific Journal
• Date: 2003

Authors

Rodrigues, C (Author) Other; Gameiro, P (Author) FCUP; Prieto, M (Author) Other; de Castro, B (Author) FCUP

Journal

Title: Biochimica et Biophysica Acta - General Subjects

Vol. 1620

Pages: 151-159

ISSN: 0304-4165

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 58 Citations

Scopus - 63 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-000-HC0

DOI: 10.1016/s0304-4165(02)00528-7

Abstract (EN): The location of isoniazid and rifampicin, two tuberculostatics commonly used for the treatment of Mycobacterium tuberculosis and Mycobacterium avium complex infectious diseases, in bilayers of dimyristoyl-L-alpha-phosphatidylcholine (DMPC) and dimyristoyl-L-a-phosphatidylglycerol (DMPG) have been studied by H-1 NMR and fluorimetric methods. Steady-state fluorescence intensity and fluorescence energy transfer studies between rifampicin and a set of functionalized probes {n-(9-anthroyloxy)stearic acids, n = 2, 12} reveal that, in both systems, isoniazid is located at the membrane surface whereas rifampicin is deeply buried inside the lipid bilayers. Steady-state fluorescence anisotropy studies performed with the probes 1,6-diphenyl-1,3,5-hexatriene (DPH) and trimethylammonium-diphenylhexa-triene (TMA-DPH), not only corroborate the above results, but also show that no changes in membrane fluidity were detected in either liposome. The H-1 NMR results, in DMPC liposomes, confirm the location of rifampicin near the methylene group of the acyl chains of the lipid bilayers.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9