Activity and location of olive oil phenolic antioxidants in liposomes

• Type: Article in International Scientific Journal
• Date: 2003

Authors

Paiva Martins, F (Author) FCUP; Gordon, MH (Author) Other; Gameiro, P (Author) FCUP

Journal

Title: Chemistry and Physics of Lipids

Vol. 124

Pages: 23-36

ISSN: 0009-3084

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 85 Citations

Scopus - 94 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-000-GBT

DOI: 10.1016/s0009-3084(03)00032-x

Abstract (EN): The antioxidant activity of hydroxytyrosol, hydroxytyrosol acetate, oleuropein, 3,4-dihydroxyphenylelenolic acid (3,4-DHPEA-EA) and 3,4-dihydroxyphenyielenolic acid dialdehyde (3,4-DHPEA-EDA) towards oxidation initiated by 2,2'-azobis (2-amidinopropane) hydrochloride in a soybean phospholipid liposome system was studied. The antioxidant activity of these olive oil phenols was similar and the duration of the lag phase was almost twice that of alpha-tocopherol. Trolox(R), a water-soluble analogue of alpha-tocopherol, showed the worst antioxidant activity. However, oxidation before the end of the lag phase was inhibited less effectively by the olive oil phenols than by alpha-tocopherol and Trolox(R). Synergistic effects (11-20% increase in lag phase) were observed in the antioxidant activity of combinations of alpha-tocopherol with olive oil phenols both with and without ascorbic acid. Fluorescence anisotropy of probes and fluorescence quenching studies showed that the olive oil phenols did not penetrate into the membrane, but their effectiveness as antioxidants showed they were associated with the surface of the phospholipid bilayer.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 14