The heterologous systems in the study of cardosin b trafficking pathways

• Type: Article in International Scientific Journal
• Date: 2011

Authors

Costa, DS (Author) FCUP; Pereira, S (Author) FCUP; Pissarra, J (Author) FCUP

Journal

Title: Plant Signaling and Behavior

Vol. 6

Pages: 895-897

ISSN: 1559-2316

Publisher: Taylor & Francis

Indexing

ISI Web of Knowledge

Scopus - 6 Citations

Other information

Authenticus ID: P-007-YPK

DOI: 10.4161/psb.6.6.15321

Abstract (EN): Cardosins are abundant in cardoon pistils and were found to accumulate in different cell compartments: cardosin A was detected in the vacuoles of stigmatic papillae and cardosin B accumulates in the extracellular matrix of the transmitting tissue. Due to the fact that cardoon system imposes some limitations to the study of processing and trafficking events, heterologous species have been employed to study cardosins trafficking pathways. Cardosin B was successfully expressed both in Arabidopsis and Tobacco plants, where it accumulated mainly in the vacuole but it was also detected in the cell wall. The glycosylation pattern of cardosin B was replicated in these systems-high-mannose type glycans. In tobacco leaves, cardosin B is transported through the Golgi in a RABD2a- dependent route, and is delivered to the vacuole via the prevacuolar compartment in a RAB-F2b-dependent pathway. © 2011 Landes Bioscience.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 3