Spectrophotometric determination of drug partition coefficients in dimyristoyl-L-alpha-phosphatidylcholine/water: a comparative study using phase separation and liposome suspensions

• Type: Article in International Scientific Journal
• Date: 2001

Authors

Rodrigues, C (Author) Other; Gameiro, P (Author) FCUP; Reis, S (Author) FFUP; Lima, JLFC (Author) FFUP; de Castro, B (Author) FCUP

Journal

Title: Analytica Chimica Acta

Vol. 428

Pages: 103-109

ISSN: 0003-2670

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 33 Citations

Scopus - 37 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-000-WE4

DOI: 10.1016/s0003-2670(00)01209-5

Abstract (EN): The partition coefficients (K-p) between lipid bilayers of dimyristoyl-L-alpha -phosphatidylcholine (DMPC) unilamellar liposomes and water were calculated for four drugs, chlordiazepoxide (a benzodiazepine), isoniazid and rifampicin (tuberculostatic drugs) and griseofulvin (an antifungal antibiotic), using spectrophotometric data and two different approaches. In one, spectrophotometric data were obtained in liposome suspensions and the values of Kp were determined from second derivative intensities. In the other, the aqueous and lipid phases were separated by filtering through a Centricon filter unit prior to spectrophotometric determination of drug concentration in each phase. Both methods provide precise and accurate values for the partition coefficient of the drugs in water (Hepes)/DMPC media. None of them presents a clear advantage in the total time needed for the determinations, but for drugs which absorb near or below 275 nm, the need to eliminate liposome scattering may favdour the phase separation method.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7