Molecular and genotypic characterization of human thyroid follicular cell carcinoma-derived cell lines

• Type: Article in International Scientific Journal
• Date: 2007

Authors

Ana Margarida Meireles (Author) Other; Ana Preto (Author) Other; Ana Sofia Rocha (Author) Other; Ana Paula Rebocho (Author) Other; Valdemar Maximo (Author) FMUP; Isabel Pereira Castro (Author) Other; Severina Moreira (Author) Other; Talia Feijao (Author) Other; Tiago Botelho (Author) Other; Ricardo Marques (Author) Other; Vitor Trovisco (Author) Other; Luis Cirnes (Author) Other; Cintia Alves (Author) Other; Sergia Velho (Author) Other; Paula Soares (Author) FMUP; Manuel Sobrinho Simoes (Author) FMUP

Journal

Title: Thyroid

Vol. 17 No. 8

Pages: 707-715

ISSN: 1050-7256

Publisher: Mary Ann Liebert

Indexing

ISI Web of Knowledge - 83 Citations

Scopus - 84 Citations

Scientific classification

FOS: Medical and Health sciences > Clinical medicine

Other information

Authenticus ID: P-004-8PQ

DOI: 10.1089/thy.2007.0097

Abstract (EN): Objective: Our aim was to characterize the molecular and genotypic profile of eight thyroid carcinoma-derived cell lines-TPC1, FB2, B-CPAP, K1, XTC-1, C643, 8505C, and Hth74-in order to use them as in vitro models of thyroid carcinogenesis. Design: We evaluated the expression of five thyroid-specific genes (Tg, TSHr, TPO, PAX8, and TTF-1) to establish the cell lineage and to assess the differentiation status of each of the cell lines. We screened for mutations in the most relevant oncogenes/tumor suppressor genes affected in thyroid carcinogenesis: RAS, BRAF, CTNNB1, and TP53 along with RET/PTC rearrangements. Considering the putative relevance in general carcinogenesis, we have also studied other molecules such as EGFR, PI3K, RAF-1, and THRB. To determine the genetic identity of the cell lines, we performed genotypic analysis. Main outcome: The panel of cell lines we have studied displayed activation of several oncogenes (BRAF, RAS, RET/PTC) and inactivation of tumor suppressor genes (TP53) known to be important for thyroid carcinogenesis. Two of the cell lines-TPC1 and FB2-shared the same genotypic profile, probably representing clones of an ancestor cell line (TPC1). Conclusion: Due to their different molecular alterations, these cell lines represent a valuable tool to study the molecular mechanisms underlying thyroid carcinogenesis. We suggest that genotypic analyses should be included as a routine procedure to guarantee the uniqueness of each cell line used in research.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9