Carcinogenic liver fluke Opisthorchis viverrini oxysterols detected by LC-MS/MS survey of soluble fraction parasite extract

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Nuno Vale (Author) FCUP; Maria Joao Gouveia (Author) Other; Monica Botelho (Author) Other; Banchob Sripa (Author) Other; Sutas Suttiprapa (Author) Other; Gabriel Rinaldi (Author) Other; Paula Gomes (Author) FCUP; Paul J Brindley (Author) Other; Jose Manuel C Correia da Costa (Author) Other

Journal

Title: Parasitology International

Vol. 62

Pages: 535-542

ISSN: 1383-5769

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 34 Citations

Scopus - 36 Citations

Scientific classification

FOS: Medical and Health sciences > Health sciences

Other information

Authenticus ID: P-008-DH6

DOI: 10.1016/j.parint.2013.08.001

Abstract (EN): Liquid chromatography in tandem mass spectrometry (LC-MS/MS) has emerged as an informative tool to investigate oxysterols (oxidized derivatives of cholesterol) in helminth parasite associated cancers. Here, we used LC-MS/MS to investigate in soluble extracts of the adult developmental stage of Opisthorchis viverrini from experimentally infected hamsters. Using comparisons with known bile acids and the metabolites of estrogens, the LC-MS data indicated the existence of novel oxysterol derivatives in O. viverrini. Most of these derivatives were ramified at C-17, in similar fashion to bile acids and their conjugated salts. Several were compatible with the presence of an estrogen core, and/or hydroxylation of the steroid aromatic ring A, hydroxylation of both C-2 and C-3 of the steroid ring and further oxidation into an estradiol-2,3-quinone. (C) 2013 Published by Elsevier Ireland Ltd.

Language: English

Type (Professor's evaluation): Scientific