Identification of T-cell epitopes in nonstructural proteins of foot-and-mouth disease virus

• Type: Article in International Scientific Journal
• Date: 2001

Authors

Blanco, E (Author) Other; Garcia Briones, M (Author) Other; Sanz Parra, A (Author) Other; Gomes, P (Author) FCUP; De Oliveira, E (Author) Other; Valero, ML (Author) Other; Andreu, D (Author) Other; Ley, V (Author) Other; Sobrino, F (Author) Other

Journal

Title: Journal of Virology

Vol. 75

Pages: 3164-3174

ISSN: 0022-538X

Publisher: American Society for Microbiology

Indexing

ISI Web of Knowledge - 85 Citations

Scopus - 85 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-007-9NH

DOI: 10.1128/jvi.75.7.3164-3174.2001

Abstract (EN): Porcine T-cell recognition of foot-and-mouth disease virus (FMDV) nonstructural proteins (NSP) was tested using in vitro lymphoproliferative responses, Lymphocytes were obtained from outbred pigs experimentally infected with FMDV, Of the different NSP, polypeptides 3A, 3B, and 3C gave the highest stimulations in the in vitro assays. The use of overlapping synthetic peptides allowed the identification of amino acid regions within these proteins that were efficiently recognized by the lymphocytes. The sequences of some of these antigenic peptides were highly conserved among different FMDV serotypes, They elicited major histocompatibility complex-restricted responses with lymphocytes from pigs infected with either a type C virus or reinfected with a heterologous FMDV, A tandem peptide containing the T-cell peptide 3A[21-35] and the B-cell antigenic site VP1 [137-156] also efficiently stimulated lymphocytes from infected animals in vitro. Furthermore, this tandem peptide elicited significant levels of serotype-specific antiviral activity, a result consistent with the induction of anti-FMDV antibodies. Thus, inclusion in the peptide formulation of a T-cell epitope derived from the NSP 3A possessing the capacity to induce T helper activity can allow cooperative induction of anti-FMDV antibodies by B cells.

Language: English

Type (Professor's evaluation): Scientific