Direct single-step surface plasmon resonance analysis of interactions between small peptides and immobilized monoclonal antibodies

• Type: Article in International Scientific Journal
• Date: 2000

Authors

Gomes, P (Author) FCUP; Giralt, E (Author) Other; Andreu, D (Author) Other

Journal

Title: Journal of Immunological Methods

Vol. 235

Pages: 101-111

ISSN: 0022-1759

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 22 Citations

Scopus - 23 Citations

Scientific classification

FOS: Medical and Health sciences > Basic medicine

Other information

Authenticus ID: P-007-943

DOI: 10.1016/s0022-1759(99)00218-5

Abstract (EN): Surface plasmon resonance (SPR) methods have been optimized to permit direct kinetic analysis of the antigenic peptide analytes interacting with immobilized monoclonal antibodies (mAbs). High reproducibility and a significant correlation between SPR and previous ELISA data on the same set of antibodies and peptides were observed. The kinetic data obtained provide further insight into the structure of the main antigenic site of foot-and-mouth disease virus (FMDV).

Language: English

Type (Professor's evaluation): Scientific