Bionanoconjugates of tyrosinase and peptide-derivatised gold nanoparticles for biosensing of phenolic compounds

• Type: Article in International Scientific Journal
• Date: 2011

Authors

Cortez, J (Author) Other; Vorobieva, E (Author) Other; Gralheira, D (Author) Other; Osorio, I (Author) Other; Soares, L (Author) Other; Vale, N (Author) FCUP; Pereira, E (Author) FCUP; Gomes, P (Author) FCUP; Franco, R (Author) Other

Journal

Title: Journal of Nanoparticle Research

Vol. 13

Pages: 1101-1113

ISSN: 1388-0764

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 18 Citations

Scopus - 21 Citations

Scientific classification

FOS: Engineering and technology > Materials engineering

Other information

Authenticus ID: P-002-THP

DOI: 10.1007/s11051-010-0099-8

Abstract (EN): Bionanoconjugates of the enzyme tyrosinase (TYR) and gold nanoparticles (AuNPs) functionalised with a peptide (CALNN) were produced in solution and characterised. The formation of stable TYR-AuNP:CALNN bionanoconjugates (BNCs) was supported by a decrease of the surface charge of the BNCs as determined by zeta-potential and an increase in hydrodynamic diameter as determined by Dynamic Light Scattering (DLS). UV/Vis studies of pH-induced aggregation revealed distinct protonation patterns for the BNCs when compared with AuNP:CALNN alone, further substantiating BNC formation. Activity studies of the BNCs for the reduction of di-phenols in solution indicated that TYR not only remains active after conjugation, but interestingly its activity in the BNCs is higher than for the free enzyme. In conclusion, AuNP:CALNN can provide a suitable platform for the immobilisation of TYR, leading to BNCs with increased enzyme activity and a wider pH working range, with promising uses in electrochemical biosensors for the detection of mono- and di-phenolic compounds.

Language: English

Type (Professor's evaluation): Scientific