Induction of somatic embryogenesis in Iris hollandica Hort. cv. 'Bronze Queen'

• Type: Article in International Scientific Journal
• Date: 2005

Authors

Fidalgo, F (Author) FCUP; Santos, A (Author) FCUP; Oliveira, N (Author) Other; Santos, I (Author) Other; Salema, R (Author) Other

Journal

The Journal is awaiting validation by the Administrative Services.

Title: JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY

Vol. 80

Pages: 135-138

ISSN: 1462-0316

Indexing

ISI Web of Knowledge - 8 Citations

Scopus - 11 Citations

Scientific classification

FOS: Agrarian Sciences > Agriculture, Forestry, and Fisheries

Other information

Authenticus ID: P-000-62G

Abstract (EN): Twin-scale explants of Iris hollandica cv. 'Bronze Queen' produced callus when cultured on a modified Murashige and Skoog medium supplemented with 9.0, 18.1, 27.2 or 36.2 muM 2,4-dichlorophenoxyacetic acid (2,4-D). Induction of embryogenic callus was obtained at 9.0 or 18.1 muM 2,4-D, while higher concentrations of the auxin (27.2 or 36.2 muM) were not effective. Embryo-like structures developed on the surface of embryogenic calli. Somatic embryo maturation was achieved when the bipolar structures produced were cultured on medium without auxin. To improve embryo germination, the developing embryos were transferred to medium supplemented with 0.95 muM abscisic acid (ABA). In this medium, mature embryos developed into normal plantlets. Histological studies confirmed that the structures exhibiting bipolar differentiation were somatic embryos. Regeneration of I. hollandica cv. 'Bronze Queen' plants through somatic embryogenesis was thus established.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 4