Specific roles of potato glutamine synthetase isoenzymes in callus tissue grown under salinity: molecular and biochemical responses

• Type: Article in International Scientific Journal
• Date: 2006

Authors

Jorge Teixeira (Author) FCUP; Susana Pereira (Author) FCUP; Filipa Queiros (Author) Other; Fernanda Fidalgo (Author) FCUP

Journal

Title: Plant Cell, Tissue and Organ Culture

Vol. 87

Pages: 1-7

ISSN: 0167-6857

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 12 Citations

Scopus - 13 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-004-H0H

DOI: 10.1007/s11240-006-9103-5

Abstract (EN): Soil salinity is an important determinant to crop distribution and productivity. Proline accumulates in response to salt stress. Glutamine synthetase (GS, EC 6.3.1.2) present in the phloem plays a major role in regulating proline content. Potato (Solanum tuberosum L. cv. Desiree) plants appear to have three nuclear GS-encoding genes: one codes for plastidial (GS2) and the other two code for cytosolic (GS1) isoenzymes. Potato plants possess a low to moderate tolerance to salinity. Three callus tissue lines tolerant to increasing levels of salinity were grown under different salt concentrations (0, 50 and 100 mM NaCl) and proline and GS activity levels were determined and transcript and polypeptide accumulation patterns were analysed. The data obtained suggests a differential involvement of each potato GS isoenzyme in the tissue response to salt stress, as a result of a differential GS isogene expression, with one GS1 gene product becoming the main responsible for the GS activity detected in high salt concentrations.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7