Selected plasma biochemistry parameters in gilthead seabream (Sparus aurata) juveniles

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Peres, H (Author) FCUP; Santos, S (Author) Other; Oliva Teles, A (Author) FCUP

Journal

Title: Journal of Applied Ichthyology

Vol. 29

Pages: 630-636

ISSN: 0175-8659

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 64 Citations

Scopus - 73 Citations

Scientific classification

FOS: Agrarian Sciences > Agriculture, Forestry, and Fisheries

Other information

Authenticus ID: P-005-0HT

DOI: 10.1111/j.1439-0426.2012.02049.x

Abstract (EN): The aim of this study was to assess plasma biochemistry parameters with the potential of being used as indicators of the nutritional status for healthy gilthead seabream juveniles. Triplicate groups of 18 seabream (body weight of 58g) were kept unfed for 24h, 7 or 14days. Nine fish per treatment were then sampled randomly for blood collection and the following parameters analyzed in the plasma using standard clinical methods: glucose; protein; triglycerides; cholesterol; calcium; magnesium; inorganic phosphorus; alkaline phosphatase (ALP); aspartate aminotransferase (AST); lactate dehydrogenase (LDH); gamma-glutamyl transferase (GGT); creatine phosphokinase (CPK); and lipase. Biochemical parameters showed lower variability among individuals than did enzymatic parameters. Plasma glucose, protein, cholesterol, calcium and inorganic phosphorus levels were inversely related to the duration of starvation. On the contrary, plasma triglycerides decreased significantly during the first week of starvation and remained stable in the second week. Plasma ALP, AST and LDH decreased significantly after 1week of starvation and then remained constant. In healthy seabream juveniles, plasma glucose, protein, cholesterol, calcium and inorganic phosphorus are responsive to starvation and may be useful indicators of the nutritional status of the animals. Indicative baseline reference values for gilthead seabream juveniles starved for 24h and held at optimum temperature are: protein, 3.74.9gdl1; cholesterol, 341407mgdl1; calcium, 13.18.0mgdl1; and inorganic phosphorus, 1014.2mgdl1. Plasma triglycerides, along with plasma enzyme activities, may be useful as indicators of short term starvation. For these parameters baseline values after 1week of starvation were: triglycerides: 138230mgdl1; ALP: 58125UL1; AST: 15127UL1; and LDH 61677UL1. Plasma glucose is only responsive to longer starvation periods, remaining relatively stable during the first week of starvation, and ranging from 59 to 196mgdl1.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7