The main external alternative NAD(P)H dehydrogenase of Neurospora crassa mitochondria

• Type: Article in International Scientific Journal
• Date: 2004

Authors

carneiro, p (Author) Other; duarte, m (Author) ICBAS; videira, a (Author) ICBAS

Journal

Title: Biochimica et Biophysica Acta - Bioenergetics

Vol. 1608

Pages: 45-52

ISSN: 0005-2728

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 23 Citations

Scopus - 22 Citations

Other information

Authenticus ID: P-000-BXB

DOI: 10.1016/j.bbabio.2003.10.004

Abstract (EN): A DNA sequence homologous to non-proton-pumping NADH dehydrogenase genes was found in the genome of Neurospora crassa encoding a polypeptide of 577 amino acid residues, molecular mass of 64,656 Da, with a putative transmembrane domain. Analysis of fungal mitochondria fractionated with digitonin indicates that the protein is located at the outer face of the inner membrane of the organelle (external enzyme). The corresponding gene was inactivated by the generation of repeat-induced point mutations. Mitochondria from the resulting null-mutant nde2 are highly deficient in the oxidation of cytosolic NADH and NADPH. A triple mutant ndel/nde2/ndi1, lacking mitochondrial alternative NAD(P)H dehydrogenases, was obtained, indicating that these proteins are not essential in N. crassa. However, crosses between the nde2 mutant strain and complex I-deficient mutants yielded no viable double mutants. Transcription of the nde-2 gene, as well as of ndi-1 (internal enzyme), is repressed in the late exponential phase of fungal growth. (C) 2003 Elsevier B.V. All rights reserved.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8