Sequence variation and mRNA expression of the TWIST1 gene in cats with mammary hyperplasia and neoplasia

• Type: Article in International Scientific Journal
• Date: 2012

Authors

Baptista CS (Author) ICBAS; Santos S (Author) Other; Laso A (Author) Other; Bastos E (Author) Other; Ávila S (Author) Other; Guedes-Pinto H (Author) Other; Gartner F (Author) ICBAS; Gut IG (Author) Other; Castrillo JL (Author) Other; Chaves R (Author) Other

Journal

Title: Veterinary Journal

Vol. 191

Pages: 203-207

ISSN: 1090-0233

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 9 Citations

ISI Web of Science

Scopus - 10 Citations

Pubmed / Medline

Scientific classification

FOS: Agrarian Sciences > Veterinary science

Other information

Authenticus ID: P-002-D8M

DOI: 10.1016/j.tvjl.2011.01.011

Abstract (EN): In humans, a germline mutation (c.309C > G) in the TWIST1 oncogene may predispose to breast cancer and its expression has been associated with tumour progression and metastasis. In this study, the feline TWIST1 gene was screened for sequence variations in 37 neoplastic and eight hyperplastic mammary gland lesions from cats. In addition, mRNA levels were examined in 15 mammary tumours and three cases of mammary hyperplasia by quantitative real-time reverse-transcriptase PCR. Feline mammary carcinomas had significantly lower levels of expression of TWIST1 mRNA than benign mammary tumours. No variations were identified in the TWIST1 coding region in feline mammary tumours and the mutation described in humans was not detected. However, two germline variants in the TWIST1 gene intron were identified in four and three carcinomas, respectively: GQ167299:g.535delG and GQ167299:g.460C > T. There was no association between these sequence alterations and TWIST1 mRNA levels.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5